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KMID : 0387420040150010077
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Korean Society of Oriental Neuropsychiatry
2004 Volume.15 No. 1 p.77 ~ p.86
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Study on the Agonistic Effect of Chunmajeongal-tang Extract to the $GABA_A/benzodiazepine$ Receptor Complex
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Kim Sung-Wook
Gong Dae-Jong
Kim Geun-Woo
Koo Byung-Su
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Abstract
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Objective:This study was performed to investigate the agonistic activity of Chunmajeongal-tang extract to the $GABA_A/benzodiazepine$ receptor complex.
Methods: Male mice and Sprague-Dawley rats were used for this experiment. Chunmajeongal-tang Prescription was extracted with 80% methanol, evaporated in vacuo and dried with freeze dryer. The agonistic activity to the GABA/ benzodiazepine receptor complex and GABA transaminase activity were measured in vitro.
Results: Chunmajeongal-tang extract inhibited dose-dependently the binding of [3H]Ro15-1788, an antagonist on GABA/benzodiazepine receptor complex, in rat cerebral cortices, showing $82.4{\pm}4.12%$ inhibition at a dose of 5.0 mg/kg. This extract inhibited dose-dependently the binding of [3H]flunitrazepam, an agonist on GABA/benzodiazepine receptor complex, in rat cerebral cortices, showing $5.6{\pm}1.24%$ inhibition. Furthermore, Chunmajeongal-tang extract inhibited the binding of [3H]flunitrazepam in the presence of GABA/NaCI with $13.2{\pm}0.44%$ inhibition, its inhibitory effect exhibited a positive GABA shift, which means that this extract activates a GABAergic neurotransmission.
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KEYWORD
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Chunmajeongal-tang, GABA/benzodiazepine receptor, sedative effect
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